Aptamer-mediated drug release

摘要

Disclosed are aptamer/drug conjugate complexes and the use of such complexes, together with a trigger compound, to inducibly release a drug. Also disclosed are methods for establishing a drug reservoir in a subject using these complexes, whereby drug may be released as needed. One example of such a complex is an aptamer/insulin conjugate complex from which insulin may be released by an innocuous, orally administrable trigger, such as quinine.

主权项

1. An aptamer complex comprising:
(i) a first trigger-binding oligonucleotide portion comprising the sequence of SEQ ID NO:12; and (ii) a drug conjugate comprising a second releasable oligonucleotide portion comprising the sequence of SEQ ID NO: 2 or SEQ ID NO: 13 linked to a drug selected from the group consisting of a pharmaceutical compound, a peptide, a protein, a peptidomimetic compound, and an imaging agent; wherein the binding of a trigger compound releases the drug conjugate from the complex, and wherein the trigger compound is selected from the group consisting of quinine and quinidine.