发明名称 |
METHODS FOR THE INTERCELLULAR TRANSFER OF ISOLATED MITOCHONDRIA IN RECIPIENT CELLS |
摘要 |
The present invention relates to methods for the intercellular transfer of isolated mitochondria in recipient cells. In particular, the present invention relates to a method for the intercellular transfer of an amount of mitochondria isolated from a population of donor cells into a population of recipient cells comprising the step of i) centrifuging the population of recipient mammalian cells in presence of the isolated mitochondria at centrifugation force ranging from 1000g to a 2000g at a temperature ranging from 1°C to 8°C and for a time ranging from 5min to 30min, ii) resting the centrifuged cells at a temperature ranging from 30°C to 40°C for a time ranging from 90min to 180min, and iii) repeating the cycling of steps i) and ii) for a sufficient number of times for reaching transfer efficiency. |
申请公布号 |
WO2016008937(A1) |
申请公布日期 |
2016.01.21 |
申请号 |
WO2015EP66190 |
申请日期 |
2015.07.15 |
申请人 |
INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE);UNIVERSITE DE MONTPELLIER;CENTRE HOSPITALIER UNIVERSITAIRE DE MONTPELLIER |
发明人 |
VIGNAIS, MARIE-LUCE;BRONDELLO, JEAN-MARC;JORGENSEN, CHRISTIAN;CAICEDO PALIZ, ANDRÉS BERNARDO |
分类号 |
A61K35/12;C12N5/00 |
主分类号 |
A61K35/12 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|