一种稳定、抗干扰能力强的血清锌检测试剂及检测方法

摘要

本发明涉及血清锌检测技术领域，特别涉及一种血清锌检测试剂，试剂R1中含有缓冲液、水杨醛肟、丁二酮肟盐、氟碳表面活性剂FSO-300、2,9-二甲基-1,10-菲咯啉、抗坏血酸、亚铁氰化钾、聚乙二醇6000、乙二醇、柠檬酸三钠、NaCl、防腐剂；试剂R2中含有缓冲液、二甲基亚砜、硝基-PAPS、氟碳表面活性剂FSO-300、抗坏血酸、亚铁氰化钾、聚乙二醇6000、乙二醇、柠檬酸三钠、NaCl、防腐剂。采用Tris缓冲液，添加稳定剂及多种抗干扰剂，可以有效去除铜、铁以及钙、镁等离子的干扰，显著改善了试剂的稳定性；并且添加的新型非离子型氟碳表面活性剂FSO-300，可促进血清锌的裂解，可防止反应体系浑浊，增强显色物质的稳定性，避免了除蛋白等预处理步骤，显著提高了试剂的灵敏度和稳定性。

主权项

一种血清锌检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：试剂R1的组分为：缓冲液_{···········································································}200mmol/L，水杨醛肟_{········································································}5mmol/L，丁二酮肟盐_{····································································}2mmol/L，氟碳表面活性剂FSO‑300_{··············································}10ml/L，2,9‑二甲基‑1,10‑菲咯啉_{··············································}3mmol/L，抗坏血酸_{·········································································}5mmol/L，亚铁氰化钾_{·····································································}1mmol/L，聚乙二醇6000_{································································}10g/L，乙二醇_{··············································································}5ml/L，柠檬酸三钠_{·······································································}12g/L，NaCl_{···················································································}9g/L，防腐剂_{············································································}0.5g/L；试剂R2的组分为：缓冲液_{···········································································}200mmol/L，二甲基亚砜_{······································································}5ml/L，硝基‑PAPS_{······································································}0.5mmol/L，氟碳表面活性剂FSO‑300_{···················································}10ml/L，抗坏血酸_{·········································································}5mmol/L，亚铁氰化钾_{·····································································}1mmol/L，聚乙二醇6000_{································································}10g/L，乙二醇_{··············································································}5ml/L，柠檬酸三钠_{·······································································}12g/L，NaCl_{···················································································}9g/L，防腐剂_{··············································································}0.5g/L。