| 发明名称 | Tolerogenic dendritic cells, method for their production and uses therof | ||
| 摘要 | The present invention relates to a tolerogenic dendritic cell population (Tr-DC) capable of generating a population of T cells having regulatory activity, method of production and uses thereof. Furthermore, soluble HLA-G promotes the differentiation of a population of T cells with regulatory activity. | ||
| 申请公布号 | US9234174(B2) | 申请公布日期 | 2016.01.12 |
| 申请号 | US200712300764 | 申请日期 | 2007.03.30 |
| 申请人 | OSPEDALE SAN RAFFAELE S.R.L.;FONDAZIONE TELETHON | 发明人 | Gregori Silvia Adriana;Roncarolo Maria Grazia;Bacchetta Rosa |
| 分类号 | C12N5/0784;C12N5/0783;C12N5/00;C12N5/02;C12N5/07;A61K35/12 | 主分类号 | C12N5/0784 |
| 代理机构 | Lucas & Mercanti LLP | 代理人 | Lucas & Mercanti LLP |
| 主权项 | 1. An in vitro method for generating a population of human tolerogenic dendritic cells (Tr-DC) comprising the steps of: a) collecting peripheral blood mononuclear cells (PBMCs) from a subject; b) isolating adherent cells from collected PBMCs; and c) exposing said isolated adherent cells at the start of culture on day 0 to an effective amount of GM-CSF, IL-4 and IL-10 to generate human Tr-DC,wherein the generated human Tr-DC have a mature myeloid phenotype and have a marker phenotype comprising: CD14+, CD11c+, CD11 b+, CD83+, CD80+, CD86+, HLA-DR+, CD71+ and CD1a−; wherein at least about 92% of the generated human Tr-DC are CD86+positive. | ||
| 地址 | Milan IT | ||