发明名称 |
Method of identifying transmembrane protein-interacting compounds |
摘要 |
<p>A method for screening a candidate compound for its ability to interact with at least one transmembrane protein comprising:
transfecting a cell with at least one nucleotide sequence encoding a protein comprising a transmembrane protein containing at least one nuclear localisation sequence (NLS) and a detectable moiety and permitting expression of the encoded protein in the cell;
contacting the cell with a candidate compound; and
determining the distribution of the expressed protein in the cell by detecting the distribution of the detectable moiety in the cell;
wherein detection of an altered distribution of the detectable moiety in the cell relative to the distribution of the detectable moiety in a control cell not contacted with the candidate compound indicates that the compound interacts with the transmembrane protein, and
wherein the wild type transmembrane protein contains an NLS.</p> |
申请公布号 |
EP2073013(B1) |
申请公布日期 |
2016.01.06 |
申请号 |
EP20090150972 |
申请日期 |
2003.04.11 |
申请人 |
OMEROS CORPORATION |
发明人 |
O'DOWD, BRIAN F.;GEORGE, SUSAN R. |
分类号 |
G01N33/68;C07K14/705;C07K14/71;C07K14/72;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/09;C12N15/85;C12Q1/02;G01N33/15;G01N33/50;G01N33/53;G01N33/543;G01N33/566;G01N33/567;G01N33/92 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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