Process for the Preparation of Multicellular Spheroids

摘要

The invention pertains to a process for the preparation of multicellular spheroids from a suspension of single cells, wherein the cells are directly derived from a biological tissue and/or from cell-containing bodily fluid. The invention is further directed to the multicellular spheroids obtained by the process according to the invention as well as to the use of the spheroids for diagnostic, screening and therapeutic purposes.

主权项

1. A process for the preparation of multicellular spheroids, comprising
a) preparing in a medium, a suspension of single cells from at least one biological tissue or cell-containing bodily fluid, b) adjusting the concentration of cells in the suspension to a concentration in the range of from 103 cells to 107 cells, c) adding 2 to 50 vol.-% of an inert matrix to the suspension of single cells, and d) incubating the suspension of single cells,wherein the suspension of single cells is directly derived from at least one biological tissue or cell-containing bodily fluid.