主权项 |
1. A method of preparing a mixture, comprising:
incubating a tissue sample from a subject with a divalent cation chelating agent until said tissue sample is separated into multiple layers, wherein said tissue sample contains a plurality of cells; and collecting one of said layers, wherein said one of said layers contains somatic stem cells that are 2 to less than 6 micrometers in size and Lgr5+. |