NOVEL MULTISPECIFIC CONSTRUCTS

摘要

Provided are novel multispecific antibody constructs and multispecific antibody drug conjugates (ADCs), and methods of using such antibodies and ADCs to treat cancer. IgG-like bispecific antibodies have different binding specificities on each arm of the antibody. They are similar in structure to monospecific IgGs in that they contain two heavy chains with VH, CH1, CH2 and CH3 regions, and two light chains with VL and CL regions.

主权项

1. A multispecific antibody comprising a first binding region having a first CL domain, and a first CH1 domain and a second binding region having a second CL domain and a second CH1 domain wherein said multispecific antibody comprises at least one amino acid substitution that alters the electrostatic charge distribution in a domain selected from the group consisting of the first CL domain, the first CH1 domain, the second CL domain and the second CH1 domain whereby the altered charge distribution promotes preferential assembly of a multispecific antibody as compared to a monospecific antibody.