Oligonucleotide primer with omega structure for detecting short-chain RNAs and use thereof

摘要

Disclosed are an oligonucleotide primer with an omega structure for detecting short-chain RNAs and the use thereof. The primer from the 5′ end to the 3′ end sequentially is: a PCR primer target region of 20-30 bases, a variable coding region of 0-50 bases, an omega stem-loop, a probe spacer of at least one base and a probe region of 4-11 bases. The length of the stem of the omega stem-loop is 4-12 paired bases, and the length of the loop of the omega stem-loop is 3-20 unpaired bases.

主权项

1. An oligonucleotide primer with omega structure for short-chain RNAs detection, characterized in that: said primer has a 5′ terminal through a 3′ terminal sequentially comprising: a PCR primer target region, a variable coding region, an omega stem-loop, a spacer region for probe and a probe region, wherein the length of said PCR primer target region is 20-30 bases, the length of said variable coding region is 0-50 bases, the stem length of said omega stem-loop is 4-12 bases the loop length of said omega stem-loop is 3-20 bases the length of said spacer region for probe is at least 1 base, and the length of said probe region is 4-11 bases, wherein the loop, PCR primer target region, variable coding region, spacer region and probe region are single stranded, and the stem is double-stranded.