Attenuated swine influenza vaccines and methods of making and use thereof

摘要

This disclosure provides attenuated swine influenza strains, particularly those produced via a reverse genetics approach, compositions comprising same, and methods of production and use thereof. The attenuated strains are engineered to encode HA proteins having additional glycosylation sites, relative to the HA proteins encoded by the corresponding virulent parental viruses. Advantageously, the attenuated influenza strains may be administered.

主权项

1. An attenuated swine influenza strain capable of providing a safe and effective immune response in a porcine animal against a virulent H3N2 swine influenza or diseases caused by H3N2 swine influenza;
wherein the attenuated strain encodes an HA protein having five additional glycosylation sites, relative to its corresponding virulent parental strain; wherein the virulent parental strain encodes an HA protein having the polypeptide sequence as set forth in SEQ ID NO: 18; and wherein the five glycosylation sites are S71N, K90N, L173T, P287T, and K294T.