NONRIBOSOMAL PEPTIDE SYNTHETASES

摘要

The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

主权项

1. A method for producing ET-743 or a metabolic intermediate thereof comprising:
growing a host cell transformed with one or more expression vectors comprising a polynucleotide encoding one or more polypeptides selected from the group consisting of SEQ ID NOs: 421, 288, 289, 290, 291, 420, 350, 423, 425, 427, 429, 431, 433 and 435 under conditions to express the one or more polypeptides and producing ET-743 or the metabolic intermediate for producing ET-743.