| 发明名称 |
CARDIOMYOCYTE DIFFERENTIATION |
| 摘要 |
This invention provides a method for testing a factor for cardiogenicity which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence and absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom, and measuring the differentiation in the presence and absence of the factor. This invention also provides a method for identifying a cardiogenic factor, which comprises differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence or absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom, and identifying the factor that affects the differentiation of human embryonic stem (hES) cells to cardiomyocytes in the presence or absence of the factor. |
| 申请公布号 |
US2015361399(A1) |
申请公布日期 |
2015.12.17 |
| 申请号 |
US201514834393 |
申请日期 |
2015.08.24 |
| 申请人 |
ES Cell International Pte Ltd. |
发明人 |
Passier Robert;Mummery Christine L. |
| 分类号 |
C12N5/077;G01N33/50 |
主分类号 |
C12N5/077 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for testing a factor for cardiogenicity said method comprising differentiating human embryonic stem (hES) cells to cardiomyocytes in the presence and absence of the factor wherein the human embryonic stem (hES) cells are cultured under a serum free condition comprising co-culture in the presence of END-2 cells or serum-free extracellular medium therefrom and measuring the differentiation in the presence and absence of the factor. |
| 地址 |
Alameda CA US |
