发明名称 |
DETECTION OF 3-(3,4-DIHYDROXYPHENYL)-LACTIC ACID (DANSHENSU) AND SALVIANOLIC ACID B IN BLOOD PLASMA AFTER ADMINISTERING A BOTANICAL EXTRACT |
摘要 |
<p>A detection method of blood plasma danshensu and salvianolic acid B of dissipating blood stasis botanical is disclosed. The method includes: (1) pretreating mammalian plasma sample: applying the plasma with medicine to small column of Waters Oasis HLB activated by methanol and water; after leaching and eluting, drying and enriching the eluent; after redissolving with mobile phase, measuring by UPLC/MS; (2) UPLC/MS measuring: UPLC condition: chromatographic column: Acquity UPLC BEH C 18 , 2.1'100mm, mobile phase A: water-acetonitrile-formic acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic acid 100:0.1 v/v; MS condition: electric spraying ion source(ESI), detecting with negative ion mode, scanning at the range of m/z 150-800. The method can be used for pharmacokinetics study of danshensu and salvianolic acid B in dissipating blood stasis botanical.</p> |
申请公布号 |
EP2157427(A1) |
申请公布日期 |
2010.02.24 |
申请号 |
EP20080748425 |
申请日期 |
2008.04.28 |
申请人 |
SHANGHAI SUNDISE CHINESE MEDICINE TECHNOLOGY DEVELOPMENT CO., LTD |
发明人 |
MA, YUEMING;WANG, TIANMING;ZHANG, YONGYU |
分类号 |
G01N30/36;G01N30/72;G01N33/15;G01N33/49;G01N33/52 |
主分类号 |
G01N30/36 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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