发明名称 |
METHODS FOR CREATING TRANSGENIC ANIMALS |
摘要 |
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
|
申请公布号 |
CA2254866(C) |
申请公布日期 |
2010.05.11 |
申请号 |
CA19982254866 |
申请日期 |
1998.03.18 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
BREMEL, ROBERT D.;CHAN, ANTHONY W.S.;BURNS, JANE C. |
分类号 |
C12N5/10;A01K67/027;C07K14/02;C12N5/02;C12N15/85;C12N15/867 |
主分类号 |
C12N5/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|