METHODS FOR IDENTIFYING ONE OR MORE BIOACTIVE GENES

摘要

The present invention relates to methods for identifying one or more bioactive genes, comprising: (a) introducing an expressible genomic DNA library derived from a first organism or a group of first organisms into a second organism, wherein said genomic DNA library is comprised in a, in said second organism, copy inducible vector; (b) growing said multitude of clones of said second organism at a low copy number of said vector and at high copy number of said vector; (c) identifying one or more clones of said second organism wherein said identification comprises identifying altered growth characteristic (s); and (d) identifying in the one or more clones of said second organism identified in step (c) one or more genes of said first organism or said group of first organisms providing the altered growth characteristic (s), thereby identifying the one or more bioactive genes.