| 发明名称 |
PRODUCTION CELL LINE ENHANCERS |
| 摘要 |
The present invention relates to discovery of the ectopic expression of EDEM2 in a production cell to improve the yield of a useful multi-subunit protein. Thus, the present invention provides for production cell lines, such as the canonical mammalian biopharmaceutical production cell—the CHO cell, containing recombinant polynucleotides encoding EDEM2. Also disclosed is a production cell containing both an EDEM2-encoding polynucleotide as well an XBP1-encoding polynucleotide. Improved titers of antibodies produced by these cell lines are disclosed, as well as the improved cell densities attained by these cells in culture. |
| 申请公布号 |
US2015353634(A1) |
申请公布日期 |
2015.12.10 |
| 申请号 |
US201514737090 |
申请日期 |
2015.06.11 |
| 申请人 |
REGENERON PHARMACEUTICALS, INC. |
发明人 |
CHEN Gang;BURAKOV Darya;DESHPANDE Dipali |
| 分类号 |
C07K16/22;C07K14/47 |
主分类号 |
C07K16/22 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A recombinant host cell comprising a recombinant polynucleotide that encodes (i) an endoplasmic reticulum degradation-enhancing alpha-mannosidase-like protein 2 (EDEM2) selected from (a) an amino acid sequence of SEQ ID NO:8 or (b) an amino acid sequence that is at least 92% identical to SEQ ID NO: 1, and (ii) a multi-subunit protein. |
| 地址 |
Tarrytown NY US |
