BIOMARKERS FOR RAPID DETECTION OF AN OCCURRENCE OF A STROKE EVENT

摘要

A diagnostic assay for detecting an occurrence of a stroke event in a mammalian subject. The assay comprises the steps of: (i) separating a plasma fraction from a blood sample collected from the mammalian subject; (ii) quantifying in the plasma fraction a L-glutamine hydroxylamine glutamyl transferase (L-GHGT) activity; (iii) quantifying in the plasma fraction a gamma glutamyl hydroxamate synthetase (GGHS) activity; (iv) adding together or alternatively calculating the combinatorial probability for the quantified L-GHGT activity and the quantified GGHS activity to produce a value for the net glutamine synthetase activity, and (v) correlating the net glutamine synthetase activity value with net glutamine synthetase activity values from healthy subjects to detect an occurrence of a stroke event in the mammalian subject. Also disclosed are kits comprising reagents and instructions for performing a diagnostic assay to detect and quantify L-GHGT activity and/or GGHS activity.

主权项

1. A diagnostic assay for detecting an occurrence of a stroke event in a mammalian subject, comprising:
separating a plasma fraction from a blood sample collected from the mammalian subject; quantifying in the plasma fraction a L-glutamine hydroxylamine glutamyl transferase (L-GHGT) activity; quantifying in the plasma fraction a gamma glutamyl hydroxamate synthetase (GGHS) activity; producing a value for glutamine synthetase activity by adding together the quantified L-GHGT activity and the quantified GGHS activity or alternatively, by estimating combinatorial probabilities of the quantified L-GHGT activity and the quantified GGHS activity; and correlating said glutamine synthetase activity value with a glutamine synthetase activity value from a healthy subject to detect an occurrence of a stroke event.