CELL CULTURE METHODS AND DEVICES UTILIZING GAS PERMEABLE MATERIALS

摘要

Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.

主权项

1. A method of culturing animal cells comprising:
a. a cell culture device comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of gas permeable, liquid impermeable material, said gas permeable material is in contact with ambient gas; and b. adding medium and animal cells into said cell culture device; and c. placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that the uppermost location of said medium is more than 2.0 cm higher than the lowest location of said medium, and said device is in a state of static cell culture.