发明名称 |
METHODS AND COMPOSITIONS FOR PCR USING BLOCKED AND UNIVERSAL PRIMERS |
摘要 |
Provided herein are methods and compositions for performing PCR with primers with blocked 3′-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Also described herein are methods and compositions to improve sensitivity of mutation-specific PCR when targeting closely-spaced mutations. |
申请公布号 |
EP2943590(A1) |
申请公布日期 |
2015.11.18 |
申请号 |
EP20140737615 |
申请日期 |
2014.01.13 |
申请人 |
UNITAQ BIO |
发明人 |
SPIER, EUGENE;GUEGLER, KARL |
分类号 |
C12Q1/68;C07H21/00;C12N15/10 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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