Methods for producing methionine by culturing a microorganism modified to enhance production of cysteine

摘要

A method of producing methionine, derivatives or precursors thereof includes culturing a modified microorganism in a culture medium comprising a source of carbon and a source of sulfur; and recovering methionine from the culture medium, wherein said modified microorganism has an increased expression of cysE gene encoding serine acetyltransferase, metH gene encoding methionine synthase and metF gene encoding 5,10-methylenetetrahydrofolate reductase compared to expression of the cysE, metH and metF genes in an unmodified microorganism.

主权项

1. A method of producing methionine or precursors thereof comprising:
culturing a modified microorganism in a culture medium comprising a source of carbon and a source of sulfur; and recovering methionine from the culture medium,
wherein said modified microorganism has an increased expression of cysE gene encoding serine acetyltransferase, of metH gene encoding methionine synthase, of metF gene encoding 5,10-methylenetetrahydrofolate reductase and of cysM gene encoding O-acetyl-sulfhydrylase, as compared to the expression of the cysE, metH, metF and cysM genes in an unmodified microorganism and wherein the methionine repressor encoded by the metJ gene is deleted or mutated in the modified microorganism.