发明名称 |
INTEGRATED SINGLE CELL SEQUENCING |
摘要 |
This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device. |
申请公布号 |
WO2015172080(A1) |
申请公布日期 |
2015.11.12 |
申请号 |
WO2015US29986 |
申请日期 |
2015.05.08 |
申请人 |
FLUIDIGM CORPORATION |
发明人 |
WEST, JASON A. A.;FOWLER, BRIAN;CHARN, TZE-HOWE;JOHNSON, CHRISTIAN F.;UNGER, MARC A. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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地址 |
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