METHODS AND COMPOSITIONS FOR DETECTING TARGET SNP

摘要

The present invention provides methods and compositions, and uses thereof, for simultaneously detecting one target SNP locus or multiple target SNP loci in a sample. In exemplary embodiments, the present invention also provides a multiplex SNP assay technique, which can simultaneously detect up to 20 SNP loci (40 alleles) with high level of specificity (e.g., >99.9%), sensitivity (e.g. 100%) and accuracy, high throughput, cost-effective and time-saving, reduced or no false-negative results. The present invention further provides certain isolated polynucleotides that can be used as primers or primer pairs in the present methods and composition for simultaneously detecting one target SNP locus or multiple target SNP loci in a sample.

主权项

1. A method for simultaneously detecting one target SNP locus or multiple target SNP loci in a sample, which method comprises:
conducting multiplex PCR using a target polynucleotide or multiple target polynucleotides in a sample as a template or templates and multiple pairs of primers for one SNP locus or multiple target SNP loci, and analyzing multiple PCR products using capillary electrophoresis, wherein said primers are designed so that the lengths of said PCR products from different SNP loci or from different alleles of the same SNP locus are sufficiently distinguishable from each other in capillary electrophoresis analysis.