HIGH-THROUGHPUT SCREENING METHOD FOR THE IDENTIFICATION OF BIOMARKERS, THERAPEUTIC TARGETS OR THERAPEUTIC AGENTS

摘要

The present invention relates to a method for screening a molecule of interest by means of nanoparticles comprising a candidate molecule, a tracer, and a single DNA tag specific to said molecule. The present invention also relates to a method for screening biomarkers of a disease and/or of a phenotype feature, more particularly of a cancer or an infection, by means of said nanoparticles. The invention finally relates to the nanoparticles as such as well as to a library of said nanoparticles.

主权项

1. A method for screening a molecule of interest comprising the following steps:
a) putting into contact cells with a library of synthetic nanoparticles, each synthetic nanoparticle comprising a candidate molecule, a tracer and a unique DNA tag specific to said candidate molecule, under conditions allowing said cells to integrate at least one of said synthetic nanoparticles, wherein said candidate molecule is localized at the surface of each nanoparticle; b) selecting the cells having integrated the tracer and having a phenotype of interest; and c) identifying as a molecule of interest, the candidate molecule that has been integrated into the cell selected in step b) by identifying the sequence of said unique DNA tag.