Automatic genechip array diagnosing apparatus

摘要

A method of diagnosing a sample with an automated genechip array for a fast diagnosis having high accuracy, where the sample is processed through lysis, separation, purification, labeling, reacting in the genechip array, taking a photo thereafter and analyzing the photo.

主权项

1. An automatic genechip array diagnosing method, comprising:
transferring a sample to a sample pretreatment area designated in a first corner of a rectangular loading tray injected with a processing solution; heating the sample pretreatment area; transferring magnetic beads and a reagent for separation and purification from a magnetic beads storing area and from a reagent storing device respectively to the sample pretreatment area with a fluid manipulation device; shaking the loading tray so as to mix the sample, the magnetic beads and the reagent to join the magnetic beads with the sample; transferring the sample joined with the magnetic beads in the sample pretreatment area to a sample purification area designated in a second corner of the loading tray with the fluid manipulation device; purifying the sample by attracting the magnetic beads with a magnetic controlling device; removing a supernatant without the magnetic beads in the sample purification area with the fluid manipulation device; adding reaction solution into the sample purification area with the fluid manipulation device and then shaking the loading tray to fully mix the magnetic beads with the reaction solution such that molecules are released after the sample and the magnetic beads are separated; transferring a solution containing the molecules in the sample purification area to a transcription and probe labeling area designated in a third corner of the loading tray with the fluid manipulation device; sucking a reaction solution into the transcription and probe labeling area with the fluid manipulation device; shaking the loading tray and heating the transcription and probe labeling area such that a reaction of transcription and labeling to the sample occurs; then transferring the molecules of the sample to a genechip reaction and imaging area designated in a fourth corner of the loading tray with the fluid manipulation device; sucking a plurality of reaction solutions into the genechip reaction and imaging area with the fluid manipulation device; then shaking the loading tray and heating the genechip reaction and imaging area such that a specific bonding is done by a plurality of probes in the genechip array for development with a reagent; obtaining a developed photo from the genechip reaction and imaging area after the bonding reaction of the molecules and the probes in the genechip array; and then discriminating, transferring or showing the photo taken to finish diagnosing the specific molecules in the sample.