Decellularisation of tissue matrices for bladder implantation

摘要

The invention provides an improved method of producing a natural, acellular matrix scaffold for subsequent use in tissue-engineered replacement of tissues such as the bladder. Decellularization is carried out on an expanded or distended bladder and the product retains the strength and compliance of natural material. The invention also provides use of the matrix scaffolds as wound healing material and to investigate tissue structure and function in vitro.

主权项

1. A method of implanting a tissue matrix in bladder reconstruction or replacement surgery comprising:
immersing a distensible membranous sac in a buffer solution at a mild alkaline pH which includes active amounts of a proteolytic inhibitor; (ii) distending the distensible membranous sac by introducing a sufficient volume of the same buffer solution into the interior cavity of the sac; (iii) continuing decellularisation of the sac by replacing and introducing fresh solutions around the exterior surface of the sac and/or into the sac interior so as to maintain distension of the sac during recellularisation; and (iv) implanting the whole decellularised distensible sac or a portion thereof into a donor.