发明名称 |
PREPARATION OF LIBRARIES OF PROTEIN VARIANTS EXPRESSED IN EUKARYOTIC CELLS AND USE FOR SELECTING BINDING MOLECULES |
摘要 |
The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules ("binders"), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders. |
申请公布号 |
WO2015166272(A2) |
申请公布日期 |
2015.11.05 |
申请号 |
WO2015GB51287 |
申请日期 |
2015.05.01 |
申请人 |
IONTAS LIMITED |
发明人 |
MCCAFFERTY, JOHN;DYSON, MICHAEL;PARTHIBAN, KOTHAI |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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