Synthetic Ligands for the Differentiation of Closely Related Toxins and Pathogens

摘要

Synthetic ligand compounds and methods of differentiating between Shiga toxin 1 and Shiga toxin 2 are disclosed herein. Another embodiment includes a kit for differentiating between Shiga toxin 1 and Shiga toxin 2. Assay systems and methods for providing an assay are also provided for herein.

主权项

1. A compound for detecting variant toxins wherein said toxin is selected from the group consisting of Shiga toxin 1 and Shiga toxin 2, the compound comprising the general formula (I): wherein: n equals 1, 2, 3, 4, 5 or 6, B=O, NH, S, SO, SO2, or P(O)R, C=NH2, COOH, biotin or derivatives thereof, and A comprises a glycoconjugate, wherein the glycoconjugate is selected from the group consisting of: wherein R comprises H, Ac or derivatives thereof; X=OH, SH, NHAc, NHCF3, NH2, NHCH(═NH)NH2 or derivatives thereof; Y=OH, NHAc, SH, NHCF3, NH2, NHCH(═NH)NH2 or derivatives thereof; Z=OH, NHAc, SH, NHCF3, NH2, NHCH(═NH)NH2 or derivatives thereof.