| 主权项 |
1. An in vitro method of analyzing whether the blood pressure of a subject is sensitive to sodium intake, the method comprising:
(a) suspending erythrocytes of the subject in one or more pairs of a first solution of about physiological osmolality and a second solution of about physiological osmolality, each first solution and each second solution comprising at least one of sodium chloride and potassium chloride, wherein for each pair of first and second solution the first solution comprises sodium chloride, and the second solution comprises sodium chloride or potassium chloride, wherein a second solution comprising sodium chloride has a sodium chloride concentration that is at least about 25 mM higher than the sodium chloride concentration of the first solution, and wherein a second solution comprising potassium chloride has a concentration of potassium chloride that is of at least essentially about the same value as the concentration of sodium chloride in the first solution; (b) allowing the suspended erythrocytes in the first and in the second solution of at least one pair of first and second solution to settle for a period of time sufficient to allow the formation of a supernatant; and (c) detecting the difference in height of the supernatant between the first and the second solution of the at least one pair of first and second solution, wherein an increased difference in height of the supernatants of the at least one pair of first and second solution, relative to a threshold value, indicates that the blood pressure of the subject is sensitive to sodium intake. |
