Identification and molecular characterisation of proteins, expressed in the <i>Ixodes ricinus </i>salivary glands

摘要

The invention relates to a new polynucleotide which encodes a polypeptide expressed in the salivary glands of ticks, more particularly the Ixodes ricinus arthropod tick, during the slow-feeding phase of the blood meal have, said polynucleotide and related polypeptide may be used in different constructions and for different applications which are also included in the present invention.

主权项

1. An isolated fusion protein which comprises an isolated polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:36 fused to a heterologous polypeptide, said isolated polypeptide having a kunitz-type-protease-inhibitor (KPI) domain, wherein the KPI domain of the polypeptide comprises a Phe at a position corresponding to position 40 of SEQ ID NO:36, a Gly at a position corresponding to position 44 of SEQ ID NO: 36; a Cys at a position corresponding to position 45 of SEQ ID NO:36, a Phe at a position corresponding to position 52 of SEQ ID NO:36, and a Cys at a position corresponding to position 58 of SEQ ID NO:36.