IMMOBILIZED BUFFER PARTICLES AND USES THEREOF

摘要

The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

主权项

1. A method for sequencing, the method comprising:
disposing a plurality of template nucleic acids into a plurality of confinement regions, a confinement region of the plurality of confinement regions in sensing relationship with a sensor of a sensor array, the sensor configured to provide at least one output signal representing a sequencing reaction byproduct; applying immobilize buffer particles in spatial relation to the plurality of confinement regions to buffer a solution without substantially buffering the plurality of confinement regions in which the plurality of template nucleic acids are disposed; introducing a nucleotide into the confinement region; and detecting an incorporation of the nucleotide onto a template nucleic acid of the plurality of template nucleic acids.