Methods and Devices for Processing Samples and Counting Cells

摘要

A method and device performing the method for estimation of cell count, such as sperm cell count, is disclosed. The device may be a kit including a cartridge configured to hold fluid, such as seminal fluid, and an instrument configured to centrifuge the cartridge. The cartridge and instrument are configured such that, during operation or centrifugation, they are securely attached to each other. The cartridge has a component with a defined cross-sectional volume. The defined cross-sectional volume is used to mark the component with markings, allowing a user of the device to read the markings and estimate cell volume and, thus, concentration. Various embodiments of the device are disclosed.

主权项

1. An apparatus comprising:
a cartridge configured to be rotated to cause sedimentation of particulates or cells in a received sample, the cartridge comprising:
a central cavity configured to hold the received sample;a sedimentation column configured to be in fluid communication with the central cavity, the sedimentation column comprising:
a channel or a second cavity with a cross-sectional area that is less than a cross-sectional area of the central cavity; anda sample directing cavity comprising a first opening connected to the central cavity and a second opening connected to the sedimentation column, the first opening comprising a first cross-sectional area and the second opening comprising a second cross-sectional area that is less than the first cross-sectional area, the sample directing cavity configured to hold a defined volume of the received sample and configured to direct cells into the sedimentation column during rotation of the cartridge.