Nanoparticle targeted drug delivery to the lungs using extra-testicular Sertoli cells

摘要

A method of delivering a compound of interest to the lungs of a subject by the intravenous injection of Sertoli cells loaded with a plurality of chitosan nanoparticles coupled with the compound of interest is provided. Testis-derived rat Sertoli cells were pre-loaded with chitosan nanoparticles coupled with or without the drug curcumin, pre-labeled with a fluorescent cell marker and then injected intravenously into the control or asthmatic mouse model host. Intact pre-loaded, pre-labeled Sertoli cells were present in the lungs at 15 minutes post-injection, appeared entrapped in the pulmonary pre-capillary vascular bed around alveolar sacs but were not present one hour post-injection although Sertoli cell label and cellular debris was. Most of the injected nanoparticle load (70%) and curcumin load (80%) was present in the lungs 15 minutes post-injection, and remained at 70% and 80%, respectively, one hour post-injection.

主权项

1. A method of delivering a compound of interest to the lungs of a subject, comprising:
providing a plurality of Sertoli cells; coupling a plurality of chitosan nanoparticles with the compound of interest; loading the Sertoli cells with the plurality of chitosan nanoparticles coupled to the compound of interest; and injecting the loaded Sertoli cells intravenously into the subject. wherein the loaded Sertoli cells locate in the lungs of the subject and are lysed, thereby delivering the compound of interest to the lungs.