发明名称 |
RECOMBINANT PLASMID DNA pFLAG-sc14D5a-Rm7 ENSURING SYNTHESIS OF HYBRID PROTEIN sc14D5a-Rm7, BACTERIA STRAIN Escherichia coli - PRODUCER OF HYBRID PROTEIN sc14D5a-Rm7, AND HYBRID PROTEIN sc14D5a-Rm7, COMBINING PROTEIN E OF TICK-BORNE ENCEPHALITIS MICE AND HAVING BIO-LUMINESCENCE ACTIVITY |
摘要 |
FIELD: bioengineering.SUBSTANCE: plasmid pFLAG-sc14D5a-Rm7 is designed, it ensures in cells Escherichia coli the synthesis of recombinant protein sc14D5a-Rm7 able to combine protein E of tick-borne encephalitis mice, and having bio-luminescence activity. Strain of Escherichia coli bacteria is created, it is producer of hybrid protein sc14D5a-Rm7 in cytoplasmic solution. The recombinant protein sc14D5a-Rm7 is produced, it is extracted from Escherichia coli cells, transformed by plasmid pFLAG-sc14D5a-Rm7, having molecular weight about 63.9 kDa; comprising single-chain antibody of human against protein E of tick-borne encephalitis mice, polypeptide GGSGGSGGSGGS and recombinant luciferase Renilla muelleri; having amino acid sequence, coded by nucleotide sequence SEQ ID NO:1, ensures identification of protein E of tick-borne encephalitis mice in range from 1 ng to 0.025 ng by method of bio-luminescence solid-phase immunoanalysis.EFFECT: production of bifunctional hybrid protein able to combine protein E of tick-borne encephalitis mice, and at same time having bio-luminescence activity.3 cl, 7 dwg, 6 ex |
申请公布号 |
RU2565545(C1) |
申请公布日期 |
2015.10.20 |
申请号 |
RU20140145690 |
申请日期 |
2014.11.13 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE NAUKI INSTITUT KHIMICHESKOJ BIOLOGII I FUNDAMENTAL'NOJ MEDITSINY SIBIRSKOGO OTDELENIJA ROSSIJSKOJ AKADEMII NAUK (IKHBFM SO RAN);FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE NAUKI INSTITUT BIOFIZIKI SIBIRSKOGO OTDELENIJAROSSIJSKOJ AKADEMII NAUK (IBF SO RAN) |
发明人 |
FRANK LJUDMILA ALEKSEEVNA;TIKUNOVA NINA VIKTOROVNA;BURAKOVA LJUDMILA PETROVNA;BAJKOV IVAN KONSTANTINOVICH;KUDRJAVTSEV ALEKSANDR NIKOLAEVICH;MOROZOVA VERA VITAL'EVNA |
分类号 |
C12N15/70;C07K19/00;C12N1/21 |
主分类号 |
C12N15/70 |
代理机构 |
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