METHOD FOR PREPARING aRNA.

摘要

The present invention provides a method for preparing aRNA to be used for gene expression analysis from an RNA sample extracted from a tissue or cell(s) fixed with a fixative, wherein, in an amplification step of the RNA sample by reverse transcription and in vitro transcription, the ratio of aminoallyl uridine 5'-triphosphate (AA-UTP) in a nucleotide reagent used in the in vitro transcription is not less than 5 mol% and less than 25 mol% with respect to the total of uridine 5'-triphosphate (UTP) and AA-UTP.