发明名称 METHOD AND SUBSTANCES FOR ISOLATING MIRNAS
摘要 A capture probe suitable for use with a method for isolating miRNAs. A method for isolating an miRNA of interest from a sample comprising the miRNA of interest comprising providing the capture probe. A method for identifying an miRNA of interest.
申请公布号 US2015284797(A1) 申请公布日期 2015.10.08
申请号 US201514744749 申请日期 2015.06.19
申请人 BIOVENTURES, INC. 发明人 Dawson Elliott P.;Womble Kristie E.
分类号 C12Q1/68 主分类号 C12Q1/68
代理机构 代理人
主权项 1. A capture probe suitable for use with a method for isolating miRNAs, the capture probe comprising: a) a first adapter segment having a first adapter segment sequence, the first adapter segment comprising a 3′ end and a 5′ end; b) a second adapter segment having a second adapter segment sequence, the second adapter segment comprising a 3′ end and a 5′ end; and c) a microRNA binding segment having a microRNA binding segment sequence, the microRNA binding segment having a 3′ end and a 5′ end; where the microRNA binding segment is substantially complementary to, and capable of hybridizing to, one or more than one microRNA of interest by Watson-Crick base pairing; where the 5′ end of the first adapter segment is connected to the 3′ end of the microRNA binding segment; and where the 3′ end of the second adapter segment is connected to the 5′ end of the microRNA binding segment.
地址 Murfreesboro TN US