主权项 |
1. A capture probe suitable for use with a method for isolating miRNAs, the capture probe comprising:
a) a first adapter segment having a first adapter segment sequence, the first adapter segment comprising a 3′ end and a 5′ end; b) a second adapter segment having a second adapter segment sequence, the second adapter segment comprising a 3′ end and a 5′ end; and c) a microRNA binding segment having a microRNA binding segment sequence, the microRNA binding segment having a 3′ end and a 5′ end; where the microRNA binding segment is substantially complementary to, and capable of hybridizing to, one or more than one microRNA of interest by Watson-Crick base pairing; where the 5′ end of the first adapter segment is connected to the 3′ end of the microRNA binding segment; and where the 3′ end of the second adapter segment is connected to the 5′ end of the microRNA binding segment. |