| 主权项 |
1. A method of forming a composite scaffold, the method comprising:
forming an aqueous scaffold system comprising a structural protein, a weak acid, water, Ca2+, HPO42−, Mg2+, Na+, K+, Cl−, SO42−, HCO3−, and a buffer system, wherein the aqueous scaffold system has an initial pH of 6.5 to 8.0 and wherein in the aqueous scaffold system: the structural protein is collagen Type I, II, Ill, or V present in an amount of about 0.1 g/L to about 5.0 g/L the Ca2+is present in an amount of about 3.0 to about 15.0 mM, the HPO42−is present in an amount of about 3.0 to about 10.0 mM the Mg2+is present in an amount of about 0.05 to about 5.0 mM, the Na+is present in an amount of about 5.0 to about 300.0 mM, the K+is present in an amount of about 0.1 to about 10.0 mM, the Cl−is present in an amount of about 5.0 to about 300.0 mM, the SO42−is present in an amount of about 0 to about 2.0 mM, the HCO3−is present in an amount of about 0.5 to about 30.0 mM; forming a calcium phosphate-structural protein composite by the simultaneous co-precipitation of calcium phosphate and structural protein from the aqueous scaffold system, wherein the calcium phosphate is precipitated on the surface of fibers of the structural protein; isolating the calcium phosphate-structural protein composite; freeze-drying the isolated calcium phosphate-structural protein composite to form a calcium phosphate-structural protein composite scaffold. |
