GENETICALLY ENGINEERED MICROBES AND METHODS FOR CONVERTING ORGANIC ACIDS TO ALCOHOLS

摘要

Disclosed herein are genetically engineered microbes. In one embodiment, a genetically engineered microbe includes a metabolic pathway for the production of an alcohol from an organic acid. For instance, a genetically engineered microbe converts acetate, butyrate, propionate, isobutyrate, valerate, isovalerate, caproate, or phenylacetate, to Ethanol, Butanol, Propanol, Isobutanol, 1-Pentanol, Isoamylalcohol, 1-Hexanol, Phenylethanol, respectively. Also provided herein are methods of using the microbes.

主权项

1. A genetically engineered microbe comprising a metabolic pathway for the production of an alcohol from an organic acid under suitable conditions, wherein the conditions comprise a temperature of at least 50° C., at least 60° C., or at least 70° C., and wherein the metabolic pathway comprises an enzyme having aldehyde ferredoxin oxidoreductase (AOR) activity and an enzyme having alcohol dehydrogenase (AdhA) activity.