摘要 |
<p>A method of expressing heterologous proteins in insect cells using recombinant baculovirus comprising a structural gene for a heterologous protein functionally attached to an insect cellular promoter, wherein the insect cellular promoter is a promoter which is not naturally functionally attached to the structural gene, such that the heterologous protein is expressed in the insect cells having the recombinant baculovirus. The heterologous protein includes proteins capable of incapacitating the insect. The invention is also directed towards expression cassettes comprising an insect cellular promoter capable of directing the expression of a heterologous protein in tissues containing the expression cassette, recombinant expression cassettes containing heterologous proteins, transplacement fragments and vectors containing recombinant expression cassettes, and recombinant baculoviruses derived therefrom.</p> |