| 摘要 |
A method of detecting protein interactions in a biological sample. Protein components in a first aliquot of a sample are labelled with a first bifunctional dye which cross-links any-interacting components. A second aliquot of said sample functions as a control sample, in which protein components are labelled with a different, mono functional dye. Thereafter, the two aliquots are mixed and all components are separated by electrophoresis. Finally, differences in luminescence of the separated dye-labelled components are detected. The two dyes should match one another with regard to charge and/or molecular weight but should emit different kinds of fluorescent light.
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