Detection of <i>Mycobacterium tuberculosis </i>complex nucleic acids

摘要

Disclosed is a method for determining the presence of Mycobacterium tuberculosis complex nucleic acids in a test sample. In particular, regions of the IS6110 preferential locus (ipl) 3′-flanking region of the Mycobacterium tuberculosis complex genome are amplified and detected. In addition, oligonucleotides that can be used as primers to amplify the ipl 3′-flanking region and probe oligonucleotides are described.

主权项

1. A kit for identifying Mycobacterium tuberculosis complex (MT complex) nucleic acid in a sample, said kit comprising:
(a) a forward oligonucleotide primer that hybridizes to a sequence of at least 15 nucleotides of nucleotide 81-99 of SEQ ID NO: 5 or the complement of a sequence of at least 15 nucleotides of nucleotides 81-99 of SEQ ID NO: 5, and (b) a reverse oligonucleotide primer that hybridizes to a sequence of at least 15 nucleotides 165-182 of SEQ ID NO: 5 or the complement of a sequence of at least 15 nucleotides of nucleotides 165-182, and further comprising an oligonucleotide probe comprising SEQ ID NO: 3 or the complement of SEQ ID NO: 3, wherein said oligonucleotide probe is labeled with a donor fluorophore and a quenching moiety.