发明名称 |
IONIC STRENGTH-MEDIATED pH GRADIENT ION EXCHANGE CHROMATOGRAPHY |
摘要 |
The present invention provides methods for analyzing compositions of polypeptides such as antibodies by ionic strength-mediated pH gradient ion exchange chromatography. In some aspects, the methods use a combination of pH gradients and ionic strength gradients to separate the polypeptide from charge variants of the polypeptide. In some aspects, the methods use a stable ionic strength to optimize the pH gradient separation window to separate the polypeptide from charge variants. Such methods are useful for analyzing polypeptide, e.g. antibodies, with a pI greater than 9 or a pI less than 7. In some aspects, the invention provides a multiproduct method for the analysis of polypeptides of varying pI's. |
申请公布号 |
EP2920586(A1) |
申请公布日期 |
2015.09.23 |
申请号 |
EP20130855528 |
申请日期 |
2013.11.15 |
申请人 |
F. HOFFMANN-LA ROCHE SA |
发明人 |
WANG, YAJUN;MORENO, GEORGE, T.;ZHANG, BOYAN;ZHANG, LIANGYI;FARNAN, DELL;PATAPOFF, TOM |
分类号 |
G01N30/34;C07K1/18;C07K16/00;G01N30/96;G01N33/50;G01N33/68 |
主分类号 |
G01N30/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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