Gene and polypeptide sequences

摘要

The present invention provides a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif:;-X1-X2-X3-X4-X5-
where X1 is phenylalanine, tryptophan, or tyrosine, X2 is isoleucine, leucine, valine, alanine or methionine, X3 is leucine, valine, alanine or methionine, X4 is serine or threonine and X5 is isoleucine, valine, alanine or methionine; and (ii) a desired protein heterologous to the leader sequence. A polypeptide of the invention may additionally comprise, as part of the leader sequence, a secretion pro sequence. The invention also provides a polynucleotide comprising a sequence that encodes a polypeptide of the invention and a cell, preferably a yeast cell, comprising said polynucleotide.

主权项

1. A polypeptide comprising:
(i) a human albumin leader sequence, the leader sequence comprising: (a) a secretion pre sequence, and (b) the following motif:-X1-X2-X3-X4-X5- wherein X1 is phenylalanine, tryptophan, or tyrosine, X2 is isoleucine, leucine, valine, alanine or methionine, X3 is leucine, valine, alanine or methionine, X4 is serine or threonine and X5 is isoleucine, valine, alanine or methionine, and wherein the human albumin leader sequence has a final amino acid position characterized as a C-terminus; and ii) a protein heterologous to the leader sequence, wherein the protein has a first amino acid connected to and adjacent the C-terminus of the human albumin leader sequence, and wherein X1, X2, X3, X4 and X5 of the motif are at positions corresponding to −20,−19,−18,−17, and −16 when the C-terminus of the albumin leader is at position corresponding to −1.