| 发明名称 | Alkaline phosphatase | ||
| 摘要 | The present invention provides AP that has a high specific activity and preferably has superior reactivity with respect to various luminescent substrates generally used for high-sensitivity immunoassay analysis. Further preferably, the present invention provides AP having a thermal stability higher than that of CIAP. The alkaline phosphatase of the present invention is derived from the genus Shewanella and has the following characteristics: (A) molecular weight: about 104,000; (B) optimum reaction pH: about 9.5; (C) stable pH range: 5.5 to 10.4; (D) thermal stability: 65° C., and (E) specific activity: 5,000 U/mg or more. | ||
| 申请公布号 | US9133446(B2) | 申请公布日期 | 2015.09.15 |
| 申请号 | US201313973557 | 申请日期 | 2013.08.22 |
| 申请人 | TOYOBO CO., LTD. | 发明人 | Aiba Hiroshi;Kishimoto Takahide;Nishiya Yoshiaki |
| 分类号 | C12N9/16 | 主分类号 | C12N9/16 |
| 代理机构 | Westerman, Hattori, Daniels & Adrian, LLP | 代理人 | Westerman, Hattori, Daniels & Adrian, LLP |
| 主权项 | 1. A polypeptide comprising an amino acid sequence having at least 90% identity to the sequence of SEQ ID NO: 2, wherein the polypeptide has an alkaline phosphatase activity, and wherein the polypeptide does not comprise the amino acid sequence of SEQ ID NO: 2. | ||
| 地址 | Osaka JP | ||