PREPARATION OF ANTIBODY OR AN ANTIBODY FRAGMENT TARGETED IMMUNOLIPOSOMES FOR SYSTEMIC ADMINISTRATION OF THERAPEUTIC OR DIAGNOSTIC AGENTS AND USES THEREOF

摘要

The present invention provides methods of preparing an antibody- or antibody fragment-targeted cationic immunoliposome or polymer complex comprising (a) preparing an antibody or antibody fragment; (b) mixing said antibody or antibody fragment with a cationic liposome to form a cationic immunoliposome or with a cationic polymer to form a polyplex; and (c) mixing said cationic immunoliposome or said polyplex with a therapeutic or diagnostic agent to form said antibody- or antibody fragment-targeted cationic immunoliposome or polymer complex. The present invention also provides cationic immunoliposome or polymer complexes produced by such methods and compositions comprising such complexes. The present invention also provides methods for treating various diseases and disorders, including cancers, by administering the complexes and compositions of the invention to a patient.

主权项

1. An antibody- or antibody fragment-targeted cationic immunoliposome complex comprising a cationic liposome that does not comprise maleimido-phenylbutyrate-lipid (MPB-lipid), an antibody or antibody fragment, and an siRNA directed against a non-tumor disease, wherein said antibody or antibody fragment is complexed with said cationic liposome but is not chemically conjugated to said cationic liposome and wherein said antibody or antibody fragment does not comprise a lipid tag, wherein said cationic immunoliposome complex is 50-400 nm in size.