Mouse having human leukemic stem cell and leukemic non-stem cell amplified therein, and method for production thereof

摘要

The invention relates to a method of selectively expanding human leukemic cells in a non-adult NOD/SCID/IL2rgnull mouse by transplanting a substance containing a leukemic stem cell derived from a human acute myelogenous leukemia patient to the mouse. In addition, the invention relates to screening for a medicament capable of eradicating leukemic stem cell (LSC), consideration of treatment methods suitable for individual patients, identification of a differentially expressed gene and the like, using a mouse with expanded human leukemic cells.

主权项

1. A method of producing a mouse having selectively expanded human acute myelogenous leukemic cells in the peripheral blood, having human leukemic stem cells located in the endosteal surface abutting murine osteoblasts, and suppressing normal hematopoiesis, comprising
transplanting a substance containing a leukemic stem cell derived from a human acute myelogenous leukemia patient into a NOD/SCID/IL2rgnull mouse that is 4 weeks old or less, and raising the mouse, wherein the raising period after transplantation of the substance is not less than 4 weeks, thereby producing a mouse having selectively expanded human leukemic cells including hCD34+hCD38− cells, and hCD34+hCD38+ cells, and hCD34− cells in the peripheral blood, having hCD34+hCD38− cells located in the endosteal surface abutting murine osteoblasts, suppressing normal hematopoiesis, and reproducing the pathology of acute myelogenous leukemia in the patient from whom the substance is derived.