METHOD OF IN SITU SYNTHESIZING MICROARRAYS

摘要

The invention provides a method of step-wise synthesizing copies of polymers of potentially different units on at least two solid carrier surfaces simultaneously in a preselected pattern, comprising providing a layered synthesis arrangement comprising a transparent first solid carrier and a second solid carrier, wherein said first and second carrier each contain an active surface to which polymer units can be applied dependent on reactions of photosensitive moieties, projecting light in a preselected pattern onto the first and second carrier surface, wherein the light passes through the transparent first solid carrier, whereby photons react with photosensitive moieties thereby activating the first surface, said pass-through light further projects onto the second carrier surface, whereby photons react with photosensitive moieties thereby activating the second surface, applying a fluid comprising a polymer unit to the first and second active surfaces and binding the polymer unit to the exposed sites of said pattern, repeating projecting and binding steps with optionally different patterns and/or polymer units, thereby synthesising polymers on said carrier surfaces; as well as means for performing said method.

主权项

1. A method of step-wise synthesizing copies of polymers of potentially different units on at least two solid carrier surfaces simultaneously in a preselected pattern, comprising the steps of
i) providing a layered synthesis arrangement comprising a transparent first solid carrier and a second solid carrier, wherein said first and second carrier each contain a surface to which polymer units can be applied depending on reactions of photosensitive moieties, ii) projecting light in a preselected pattern onto the first carrier surface, whereby photons react with photosensitive moieties thereby activating the first surface, wherein the light passes through the transparent first solid carrier, and further projects onto the second carrier surface, whereby photons react with photosensitive moieties thereby activating the second surface, iii) applying a fluid comprising a polymer unit to the first and second activated surfaces and binding the polymer unit to the exposed sites of said pattern, iv) repeating steps ii) and iii) with optionally different patterns and/or polymer units, thereby synthesising polymers on said carrier surfaces.