Method for optimization of quantitative video-microscopy and associated system

摘要

Embodiments of the present invention are directed to a method for determining an amount of a plurality of molecular species in a sample, each molecular specie being indicated by a dye. According to one embodiment, the amount of a plurality of molecular species is determined by acquiring a plurality of images of the sample, determining an amount of each molecular specie, as indicated by a respective dye, for each pixel at each corresponding pixel location in the plurality of images, and refining the amount of a plurality of molecular species at one or more pixel locations in the plurality of images. Associated systems and computer program products are also provided.

主权项

1. A method for determining an amount of a plurality of molecular species in a sample, each molecular specie being indicated by a dye, said method comprising:
acquiring a plurality of images of the sample using an image acquisition device; determining an amount of each molecular specie, as indicated by a respective dye, for each pixel at each corresponding pixel location in the plurality of images, using a processor in communication with the image acquisition device; and refining the amount of a plurality of molecular species at one or more pixel locations in the plurality of images, wherein refining comprises determining an amount of dye concentrations that are present in the sample in a significant amount, determining a number of subsets of wavelength filter combinations, testing each of the subsets of wavelength filter combinations according to an optimality factor, and determining an optimal wavelength filter combination, and wherein testing each subset of wavelength filter combinations comprises multiplying a hypervolume of an absorption coefficient matrix and a hypervolume of a transpose of the absorption coefficient matrix specific to a particular subset of wavelength filter combinations.