MULTIPLEX ANALYSIS OF STACKED TRANSGENIC PROTEIN

摘要

FIELD: chemistry.SUBSTANCE: invention relates to biotechnology, namely to method of identifying presence of two or more proteins of interest with known amino acid sequences in sample of vegetable origin (versions) and method of preserving genotype of transgenic plant variety. Methods include cleavage of all proteins from sample of vegetable origin with sequence-specific protease with obtaining peptides. Peptides are separated in one step in device for liquid chromatography - mass spectrometry (LC-MS). Peptides are ionised in LC-MS device. Simultaneous obtaining of mass-spectral data for peptides in LC-MS device is performed. Said simultaneous mass-spectral data for peptides with expected mass-spectral data from proteins of interest are compared with known amino acid sequences. Determination of presence or absence of proteins of interest is realised.EFFECT: claimed invention makes it possible to detect presence of two or more proteins of interest of vegetable origin with high efficiency.17 cl, 10 dwg, 3 tbl, 7 ex