Process for demineralization of bone matrix with preservation of natural growth factors

摘要

A demineralized bone matrix is produced by a process in which a bone body is placed in a first processing solution comprising an acid to demineralize the bone body. The bone body is periodically removed from the first solution at specific time intervals to perform at least one test, such as a compression test, on a mechanical property of the bone body. When the test yields a desired result, the bone body is exposed to a second processing solution that is less acidic than the first, thus minimizing the exposure of the bone body to the harsh acidic conditions of the demineralization phase of the process.

主权项

1. A method for producing a demineralized cortical bone matrix, the method comprising:
placing a cortical bone body in a first processing solution comprising an acid at a pH of greater than about pH=0 to demineralize the cortical bone body; periodically removing the cortical bone body from the first processing solution at specific time intervals to perform a bending test on the cortical bone body, wherein the test comprises applying a bending force in a range from about 10 g-force/cm2 to about 4000 g-force/cm2, wherein the bending force does not cause the cortical bone body to fracture; and after the cortical bone body bends without fracturing, exposing the cortical bone body to a second processing solution.