Digitally enhanced microscopy for multiplexed histology

摘要

A multispectral imaging system (100) for imaging a specimen located on a microscope slide (134) comprise: an imaging apparatus (112) including an illuminator (140) with a plurality of different color light sources (180a-d) for sequentially producing light for sequentially illuminating the specimen, an image capture device (120) for capturing a plurality of specimen images each corresponding to the specimen being exposed to light from a respective one of the light sources, and a processing device (122) configured to produce contrast enhanced color image data and/or spectrally deconvolved image data based on the captured specimen images; at least one lens positioned along an optical path extending from the specimen to the image capture device; and a display (114) in communication with the imaging apparatus and configured to display contrast enhanced output (144) and/or spectrally deconvolved output (144) of the specimen based on the contrast enhanced color image data and/or spectrally deconvolved image data from the processing device.